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What version of IBM RPA are you using?
In some previous versions, it was not possible to use queues when the server and the client were in different subnets, another possible cause is that the client installer has not installed MSMQ and to fix this you can follow the instructions on this page:https://www.ibm.com/docs/en/rpa/23.0?topic=ic-optional-enabling-msmq-active-directory-domain-services-integration