Hi,
as long as you don't power-off both nodecanisters it is possible to remove the USB sticks from the controllers.
During startup of a controller the stored encryption key is required to unlock the access to the encrypted data. During a restart/reboot of a single nodecanister, the canister is requesting the key from its partner canister.
Storage Virtualize version 9.1.0 provide the possibility to implement encryption based on an internal key management without having the need for USB sticks or external key servers.