Hi Khushaboo,
Can you share the pool settings for this connection (min, max etc.)
When min is set to 1 you might encounter a stale connection remaining in the pool which is not useable, but IS is not able to create new connections as there is still the stale one available.
Try to set min to 0 in this case and reenable the connection.
IS will destroy the stale connection in this case and then create a new one.
Regards,
Holger
#webMethods#Integration-Server-and-ESB